GST anticorps

N° du produit ABIN964661

N° du produit (Fournisseur): 200-101-c45

Cet anticorps Chèvre Polyclonal détecte spécifiquement GST dans WB, ELISA et IHC. Il présente une réactivité envers Schistosoma japonicum.

Aperçu rapide pour GST anticorps (ABIN964661)

Antigène: GST (Glutathione S Transferase (GST))

Reactivité: Schistosoma japonicum

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp GST est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Détail du produit anti-GST anticorps

N° du produit (Fournisseur): 200-101-c45

Fournisseur: Rockland

Fonction: GST Antibody

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum].

Attributs du produit: Synonyms: goat anti-GST antibody, GST, Glutathione-S-Transferase

Purification: Anti-GST is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Glutathione-S-Transferase [Schistosoma japonicum]

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-GST antibody has been tested by ELISA and western blot. This product is assayed against 1.0 μg of Glutathione-S-Transferase [Schistosoma japonicum] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.  A working dilution of 1:3,000 to 1:15,000 of the reconstitution concentration is suggested for this product. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:1000 - 1:5000

ELISA Dilution: 1:200 - 1:1000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store GST antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur GST

Antigène: GST (Glutathione S Transferase (GST))

Autre désignation: GST

Sujet: Background: GST antibody recognizes the GST tag. The glutathione S-transferase (GST, previously known as ligandins) family of enzymes are composed of many cytosolic, mitochondrial, and microsomal (now designated as MAPEG) proteins. GSTs are present in eukaryotes and in prokaryotes, where they catalyze a variety of reactions and accept endogenous and xenobiotic substrates. Members of the GST superfamily are extremely diverse in amino acid sequence, and a large fraction of the sequences deposited in public databases are of unknown function.] The Enzyme Function Initiative (EFI) is using GSTs as a model superfamily to identify new GST functions. Anti-GST antibody is ideal for investigators involved in epitope research.